On screen
Cast
136 credited performers
Nick Kroll
Nick Birch / Maury the Hormone Monster / Coach Steve / Lola Skumpy / Rick the Hormone Monster (voice)
John Mulaney
Andrew Glouberman (voice)
Jessi Klein
Jessi Glaser (voice)
Jason Mantzoukas
Jay Bilzerian (voice)
Maya Rudolph
Diane Birch / Connie the Hormone Monstress (voice)
Fred Armisen
Elliot Birch (voice)
Jordan Peele
Ghost of Duke Ellington (voice)
Andrew Rannells
Matthew (voice)
Jenny Slate
Pharmacist / Caitlin (voice)
Joe Wengert
Caleb / Pornscape Residents (voice)
Ayo Edebiri
Missy Foreman-Greenwald / Mosaic Missy (voice)
June Diane Raphael
Devin (voice)
Richard Kind
Marty Glouberman (voice)
Paula Pell
Barbara Glouberman / Lola's Couch Pillow (voice)
Neil Casey
(voice)
Jessica Chaffin
Shannon Glaser (voice)
Jon Daly
Judd Birch / Pumbaa (voice)
Jak Knight
DeVon
Chelsea Peretti
Monica Foreman-Greenwald (voice)
Seth Morris
Character not listed
Mark Rivers
Michael Stipe Tampon (voice)
Gil Ozeri
Wiggles (voice)
Nathan Fillion
Nathan Fillion (voice)
Kat Dennings
Leah Birch (voice)
Gina Rodriguez
Gina Alvarez (voice)
David Thewlis
Shame Wizard (voice)
Thandiwe Newton
Mona (voice)
Heather Lawless
Character not listed
Maria Bamford
Tito the Anxiety Mosquito (voice)
Brandon Kyle Goodman
Character not listed
Mark Duplass
Val Bilzerian (voice)
Whitmer Thomas
Camden (voice)
Paul Scheer
Character not listed
Ali Wong
Ali (voice)
Zachary Quinto
Aiden (voice)
Stephanie Beatriz
Lulu (voice)
Emily Altman
Character not listed
Chloe Fineman
Character not listed
Rob Huebel
Mr. Lizer (voice)
Natasha Lyonne
Ms. Dunn (voice)
Gary Cole
Edward MacDell (voice)
Jean Smart
Depression Kitty (voice)
Jack McBrayer
Pubic Hair #1 (voice)
John Gemberling
Tyler the Hormone Monster / Dot #1 (voice)
Craig Robinson
End Credits Singer (voice)
Michaela Watkins
Cantor Dina Reznick (voice)
Fran Gillespie
Girl (voice)
Kristen Bell
Uninterested Girl (voice)
Ashley London
Kitty / DNA Expert / Pornscape Residents (voice)
Zach Woods
Daniel (voice)
Rosa Salazar
Ms. Benitez (voice)
Quinta Brunson
Quinta (voice)
Ayden Mayeri
Gabrielle / Sad Boy #2 (voice)
Andy Daly
Dr. Wendy Engle (voice)
Bobby Cannavale
Gavin the Hormone Monster (voice)
John Oliver
Harry (voice)
Seth Rogen
Seth Goldberg (voice)
Lena Waithe
Lena (voice)
Julie White
Kimberly (voice)
Sterling K. Brown
Michael Angelo (voice)
Crissy Guerrero
(voice)
Kristen Wiig
Jessi's Vagina (voice)
Megan Thee Stallion
Megan the(e?) Hormone Monstress (voice)
Patrick Page
Character not listed
Juliet Mills
Rita St. Swithens (voice)
Ileen Goldsmith
Jessi Glaser - singing double / Judd's Girlfriend (voice)
Brendan McCreary
Freddie Mercury (singing voice)
Carlos Alazraqui
Gustavo (voice)
Julie Klausner
Cherry Marashina (voice)
Chris Redd
(voice)
Josie Totah
Natalie (voice)
Joel Kim Booster
Charles Lu (voice)
Judd Hirsch
Lewis Glouberman (voice)
Beck Bennett
Owen (voice)
Billy Porter
Ocean (voice)
J Valerione
B-High Qee-Choi Member (voice)
Keke Palmer
Character not listed
Aidy Bryant
Emmy (voice)
Rosie Perez
Character not listed
Mitra Jouhari
Character not listed
Pamela Adlon
Sonya the Lovebug (voice)
Jon Hamm
Scallops (voice)
Jon Gabrus
Movie Theater Customer (voice)
Anna Graves
Mrs. Albert (voice)
Nasim Pedrad
Fatima (voice)
Mae Whitman
Tallulah Levine (voice)
Chrissy Guerrero
Choir Girl (voice)
Philip Quinaz
Tanya Alvarez (voice)
Harvey Fierstein
Older Guy Town Resident (voice)
Jerry Minor
Man #3 / Singing Fish (voice)
Piotr Michael
Siamese Twin #2 (voice)
Marisa Lark Wallin
Reese's Wertherspoon / Woman (voice)
Martin Short
Gordie (voice)
David Cross
Skip Glouberman (voice)
Carol Kane
Menopause Banshee / Old Woman (voice)
Tawny Newsome
(voice)
Retta
Duke's Mother (voice)
Wanda Sykes
Ghost of Harriet Tubman (voice)
Jaboukie Young-White
(voice)
Rob Riggle
Sgt. Adderall (voice)
Bobby Berk
Bobby Berk (voice)
Karamo Brown
Karamo Brown (voice)
Tan France
Tan France (voice)
Antoni Porowski
Antoni Porowski (voice)
Jonathan Van Ness
Jonathan Van Ness (voice)
Anna Konkle
Izzy (voice)
Maya Erskine
Misha (voice)
Alia Shawkat
Roland (voice)
Paul Giamatti
Andrew’s Shit (voice)
Adam Scott
Mr. Dylan Keating (voice)
Hugh Jackman
himself (voice)
Kumail Nanjiani
himself (voice)
Jemaine Clement
Simon Sex (voice)
Kristen Schaal
Bernie Sanders (voice)
Somali Rose
Missy’s Overalls
Lupita Nyong'o
Asha (voice)
Miranda Tapsell
Kara (voice)
Lin-Manuel Miranda
Puerto Rican Pubic Hair (voice)
Randall Park
Pete Doheny (voice)
Maitreyi Ramakrishnan
Marissa (voice)
Ava Lalezarzadeh
Nazanin (voice)
Don Cheadle
Don Cheadle (voice)
Padma Lakshmi
Priya (voice)
Zach Galifianakis
Gratitoad (voice)
Jeff Goldblum
The Apple Brooch
Steve Buscemi
Character not listed
Skyla I'Lece
Character not listed
Katie Kreshek
Character not listed
Violet Rivers
Character not listed
Larry Owens
Character not listed
Holly Hunter
Character not listed
James III
Character not listed
Franny London
Character not listed
Cynthia Erivo
Missy's Vagina (voice)
Andrew Goldberg
(voice)
Eliseo Román
Eliseo Román (voice)