On screen
Cast
28 credited performers
Josh Keaton
Peter Parker / Spider Man (voice)
Lacey Chabert
Gwen Stacy (voice)
Joshua LeBar
Flash Thompson / Car Thief #1 (voice)
Grey DeLisle
Sally Avril / Erin (voice)
Alanna Ubach
Liz Allan (voice)
Vanessa Marshall
Mary Jane Watson / Hermia (voice)
James Arnold Taylor
Patch / Homunculi (voice)
Deborah Strang
May Parker (voice)
Clancy Brown
Alex O'Hirn / Rhino / George Stacy (voice)
Alan Rachins
Norman Osborn (voice)
Phil LaMarr
Rand Robertson / Fancy Dan (voice)
Ben Diskin
Eddie Brock (voice)
Daran Norris
J. Jonah Jameson / John Jameson (voice)
Kevin Michael Richardson
Tombstone (voice)
Peter MacNicol
Otto Octavius / Doctor Octopus (voice)
John DiMaggio
Flint Marko / Hammerhead (voice)
Dee Bradley Baker
Curt Connors (voice)
Cree Summer
Glory Grant / Cobweb / Homunculus #2 (voice)
Crispin Freeman
Electro / Clerk (voice)
Robert Englund
Vulture (voice)
Dorian Harewood
Doc Bromwell (voice)
Tricia Helfer
Felicia Hardy / Black Cat (voice)
Miguel Ferrer
Silvermane (voice)
Eric Vesbit
Kraven (voice)
Ed Asner
Ben Parker (voice)
Danny Trejo
Ox (voice)
Stan Lee
Stan (voice)
Steve Blum
Dilbert Trilby / Seymour O'Reily / Green Gobilin (voice)