On screen
Cast
35 credited performers
Neil Patrick Harris
Peter Parker / Spider-Man (voice)
Lisa Loeb
Mary Jane Watson (voice)
Ian Ziering
Harry Osborn (voice)
SuChin Pak
Alison Tomita (voice)
Angelle Brooks
Indira 'Indy' Daimonji (voice)
Ed Asner
Officer Barr (voice)
Keith Carradine
J. Jonah Jameson (voice)
Rino Romano
Doug's friend (voice)
Ethan Embry
Max Dillon (voice)
Julie Nathanson
Sally Johnson (voice)
Virginia Madsen
Silver Sable (voice)
Michael Dorn
Kraven the Hunter (voice)
James Marsters
Sergei (voice)
Clancy Brown
Policeman (voice)
Jeffrey Combs
Dr. Zellner (voice)
Karen Maruyama
Head Protestor (voice)
Jeremy Piven
Roland Gaines (voice)
Kathy Griffin
Roxanne Gaines (voice)
Jennifer Hale
Female Reporter (voice)
Michael Clarke Duncan
Wilson Fisk / The Kingpin (voice)
Stan Lee
Frank Elson (voice)
Rob Zombie
Dr. Curt Connors / The Lizard (voice)
Gina Gershon
Shikata (voice)
John C. McGinley
Richard Damien (voice)
Jane Lynch
Oscorp Executive (voice)
Tara Strong
Christina (voice)
Devon Sawa
Flash Thompson (voice)
Keith David
Agent Mosely (voice)
Eve
Cheyenne Tate / The Talon (voice)
Brendan Connolly
Aleksei (voice)
Jamie Hanes
Special Agents Roberts (voice)
Keith Szarabajka
Wesley (voice)
Harold Perrineau
Louis Wyler / Turbo Jet (voice)
Kevin Michael Richardson
Security Guard (voice)
James Horan
Henchman (voice)