On screen
Cast
19 credited performers
Fay Compton
Mary Stuart
Gerald Ames
Bothwell
Ivan Samson
Lord Dudley
John Stuart
George Douglas
Artwork unavailable
Ellen Compton
Queen Elizabeth
Artwork unavailable
Lionel d'Aragon
Moray
Harvey Braban
Ruthven
Irene Rooke
Catherine de Medici
Artwork unavailable
Donald Macardle
Francis II
René Maupré
Rizzio
Artwork unavailable
Ernest A. Douglas
Cardinal
Artwork unavailable
Sydney Seaward
Lord Douglas
Artwork unavailable
Edward Sorley
John Knox
Betty Faire
Mary Beaton
Dorothy Fane
Mary Beaton
Artwork unavailable
Nancy Kenyon
Mary Fleming
Artwork unavailable
Julie Hartley-Milburn
Mary Seaton
Basil Rathbone
Undetermined Subordinate Role
Jack Cardiff
Character not listed